Table 1.
Commercially available molecular methods for H. pylori antibiotics resistance.
| Product name | Seeplex | ClariRes | GENECUBE | HelicoDR | |
|---|---|---|---|---|---|
| Manufacturer (Country) | Seegene (Korea) | Ingenetix (Austria) | TOYOBO (Japan) | Hain Life Science (Germany) | |
| Assay technique | Dual priming oligonucleotide PCR | Real-time PCR using biprobe | Real-time PCR using quenching probe | DNA strip genotyping test combining PCR and hybridization | |
| Time-to-result | 4 h | 30 min | 6 h | ||
| Target gene | 23S rRNA | 23S rRNA | 23S rRNA | 23S rRNA | gyrA |
| Detectable mutation | A2142G, A2143G | A2142G, A2143G | A2142G, A2143G | A2142G, A2143G | 87 (Asn to Lys) |
| A2142C | A2142C | 91 (Asp to Gly, Asn, or Tyr) | |||
| H. pylori detection | Sensitivity 97.7% | Sensitivity 100% | Sensitivity 100% | ||
| Specificity 83.1% | Specificity 98–100% | Specificity 100% | |||
| (Reference test: culture) | (Reference test: culture, RUT, histology) | (Reference test: Hp -IgG, RUT, UBT) | |||
| Comparison with direct sequencing | Sensitivity 100% | Sensitivity 94.9% | Sensitivity 98.2% | ||
| Specificity 100% | Specificity 87.1% | Specificity 80.0% | |||
| Comparison with susceptibility test | Concordant rate 95.3% | Sensitivity 82-100% | Sensitivity 94% | Sensitivity 87% | |
| (41/43) | Specificity 100% | Specificity 99% | Specificity 98.5% | ||
UBT: 13C-urea breath test, Hp-IgG: serum anti-H. pylori -IgG, RUT: rapid urease test.