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. 2014 Sep 16;12:35. doi: 10.1186/s12951-014-0035-7

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© Fenollosa et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Particle localization into SK-BR-3 cell lines. SK-BR-3 (cell membrane labeled in blue) treated with PSiP-HER-2 particles (presented in green) for 24 h, showing the particles inside (arrow) and, outside (dashed arrow) the cells and at the cell membrane (opened arrow). (A) Orthogonal view in the 3 planes (X/Y, X/Z and Y/Z) of the particles pointed at the intersection of the X and Y axeis. As seen from all planes, the particles are surrounded by the cell membrane. (B) Confocal images of the cells in the transmission channel, the cell membrane and PSiP-HER-2 in fluorescent channels, and overlay of all channels. PSiP-HER-2 were labeled with DyLight 488.