Fig. 1.

a qRT-PCR assay of JARID1B levels in normal ovaries (NO), benign ovarian tumor (BEOT), and ovarian carcinoma (EOC) tissues. GAPDH serves as internal control. b The top panel presents the Western blotting analysis of JARID1B expression in normal ovaries and in ovarian carcinoma tissues. Protein samples obtained from frozen normal ovaries (NO), benign ovarian tumor (BEOT), and ovarian carcinomas (EOC) were analyzed by SDS-PAGE followed by immunoblotting with antibody against JARID1B. The levels of GAPDH were used as an internal control. The bottom panel presents the histogram of pooled data from fresh normal ovaries (NO; n = 20), benign ovarian tumor (BEOT; n = 20), and ovarian carcinomas (EOC; n = 45). The expression of JARID1B was increased both in ovarian carcinomas compared with fresh normal ovaries. Each protein samples was repeated for three times