Fig. 1.

The relationship between the promoter methylation of DACH1 and DKK1 and normalized gene expression (transcript level). Normalized level of transcript is expressed as the ratio calculated from the formula: Ratio (reference gene/target gene) = 2 ^{Ct (reference) − Ct (target)}, where TBP is the reference gene and either DACH1 or DKK1 is the target gene. Each bar represents the range of results obtained for all the samples in the respective groups with the mean value marked with the rectangle. M gene promoter methylation, U complete lack of gene promoter methylation