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. Author manuscript; available in PMC: 2015 Sep 4.
Published in final edited form as: Mol Cell. 2014 Aug 21;55(5):791–802. doi: 10.1016/j.molcel.2014.07.012

Figure 7.

Figure 7

CHART enriches proteins associated with NEAT1 and MALAT1 and reveals mechanistic insights into lncRNA function. (A) Western blot of NEAT1 and MALAT1 CHART-enriched material for the paraspeckle components PSPC1 and PSF, the nuclear speckle component SRSF1, and a chromatin-associated protein, histone H3. (B) Table indicating number of known nuclear speckle and paraspeckle proteins enriched ten-fold over input for the indicated CHART conditions. (C) Venn diagram depicting overlap between proteins associated with NEAT1 and MALAT1. (D) Western blot of NEAT1 and MALAT1 CHART-enriched material for PURA and ESRP2 protein. (E) Model of NEAT1 and MALAT1 binding to trans genomic sites. (F) Model depicting preferential localization of NEAT1 RNA at the TSS and TTS and MALAT1 RNA at the TTS. See also Table S6 and Figure S7.