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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: Microsc Microanal. 2011 Jun 24;17(4):614–617. doi: 10.1017/S1431927611000535

Figure 2.

Figure 2

Clearing allows visualization of intact 3D organization within tissues. Fixed adult rat kidney tissue was imaged using clearing plus increase of laser intensity with depth. (a) Single xz-section of rat kidney stained with FITC-conjugated-lens culinaris agglutinin. Rendered volume, 160 µm total depth (b, c), of rat kidney stained with FITC-conjugated-lens culinaris aggutinin (blue), vimentin labeled podocytes (white), and Hoechst labeled nuclei (yellow). (b) The surface of whole volume shows a network of kidney tubules. Superficial planes removed from panel b show the glomerulus within the network of tubules (c). (b, c) Bar = 20 µm.