Figure 1.
Conditional Tcf4 deficiency in CD11c+ cells reduces tissue pDC levels and decreases atherosclerosis. Ldlr−/− mice transplanted with Tcf4-WT or Tcf4-cKO BM were fed a HFD for 8 weeks. A-C. Lymphoid tissue and blood levels of pDCs (A), B220lo cDCs (B) and cDCs (C). See methods for gating strategy. Results representative of 2 separate experiments with similar results. D. Terminal weights and serum total cholesterol levels. E. Representative images of oil red O-stained aortic root lesions (bar represents 100 μm). Quantification of total plaque area at the aortic root in 10 serial sections beginning at the start of the aortic valves (0 μm). Data represents mean ± S.E. at each position (n=10/group). Statistical significance determined by two-way ANOVA. F. Quantification of plaque CD3+ cells/mm2 in the aortic root. For each figure, significant differences between groups are indicated by p values.