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. Author manuscript; available in PMC: 2016 Jan 7.
Published in final edited form as: Neuron. 2015 Jan 7;85(1):173–189. doi: 10.1016/j.neuron.2014.12.023

Figure 6. Phosphorylation of SynGAP Regulates Spine Enlargement and GluA1 Trafficking during LTP.

Figure 6

(A and B) (A) Spine enlargement and (B) GluA1 trafficking upon LTP with or without SynGAP ([A1] and [B1]) Control: (shRNA-Ctrl), ([A2] and [B2]) Knockdown of SynGAP: (shRNA-SG#5), and ([A3] and [B3]) Knockdown of SynGAP rescued with WT: shRNA-resistant SynGAP WT rescue. ([A4] and [B4]) Knockdown of SynGAP rescued with phospho-deficient SynGAP 2SA; ([A5] and [B5]) Knockdown of SynGAP rescued with phospho-mimetic SynGAP 2SD. N = 7 independent experiments/neurons that contain 70, 66, 68, 72, and 78 spines in total, respectively. Two-way ANOVA followed by Tukey’s post hoc test was performed (shRNA+Rescue F(4, 90) = 68.76 [A]/73.30 [B], p < 0.001 [A, B]; Time F(2, 90) = 22.54 [A]/22.75 [B], p < 0.001 [A, B]; Interaction F(8, 90) = 8.88 [A]/10.90 [B], p < 0.001 [A, B]). Scale bar, 2 μm. Error bars indicate ± SEM.