(A) γH2AX immunoprecipitation for DNA double strand breaks at enhancer sequences resident in the Bcl6 (left), Cd38 (middle), and Klf6 (right) loci in wild-type, Exosc10COIN/LacZ, and Exosc3COIN/COIN ES cells following ionizing radiation treatment. (B) DNA/RNA hybrid immunoprecipitation at Ncoa3 (left), Cd38 (middle), and Klf6 (right) enhancers in wild-type, Exosc10COIN/LacZ, and Exosc3COIN/COIN ES cells. (C) Immunoprecipitation for heterochromatin marker H3K9me2 at enhancer sequences resident in the Bcl6 (left), Cd38 (middle), and Klf6 (right) loci in wild-type, Exosc10COIN/LacZ, and Exosc3COIN/COIN ES cells. (D) Immunoprecipitation for heterochromatin marker HP1γ at enhancer sequences resident in the Bcl6 (left), Cd38 (middle), and Klf6 (right) loci in wild-type, Exosc10COIN/LacZ, and Exosc3COIN/COIN ES cells. Each plot is a representation of three independent experiments performed (*P<0.05; **P<0.01 by t-test). See Figure S4.