AC treatment induces Abs that predominantly recognize PC and MDA Ags in multiplex Ag arrays. Binding of IgM is shown with replicate autoantigen microarrays, with mAbs, T15 IgM, or isotype control (at concentrations in ng/ml as shown) or adult B6 sera at 1/200, unless otherwise indicated. Sera were obtained from individual naive age-matched adult B6 mice or at day 16 from mice that received three infusions of saline control treatment (PBS A and PBS B), or apoptotic thymocytes on days 0, 7, and 14 (Apoptotic A and Apoptotic B). Results are representative of two independent experiments.