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. 2015 May 11;6:7007. doi: 10.1038/ncomms8007

Figure 3. Autophagosome–lysosome fusion is active in V-ATPase-depleted cells.

Figure 3

(a) Confocal images of larval fat body cells co-expressing mCherry-Atg8a (red), the lysosomal marker LAMP1-GFP (green) and the indicated RNAi constructs under fed or 4 h starved conditions. Insets illustrate the presence of autolysosomes indicating fusion between autophagosomes and lysosomes. Autolysosomes can be identified as mCh-Atg8a-positive structures surrounded by LAMP1-GFP in starved controls and fed or starved V1H-depleted cells. Genotypes: control: Cg-GAL4 UAS-LAMP1-GFP UAS-mCh-Atg8a/+. Syx17 RNAi: Cg-GAL4 UAS-LAMP1-GFP UAS-mCh-Atg8a/ UAS-Syx17-dsRNA. V1H RNAi: Cg-GAL4 UAS-LAMP1-GFP UAS-mCh-Atg8a/ UAS-V1H-dsRNA. Nuclei are labelled in blue with DAPI; scale bars, 10 μm. (b) Quantitation of LAMP1-GFP and mCh-Atg8a co-localization as in a. Pearson's correlation coefficient Rr was calculated using ≥10 independent samples for each genotype; error bars mark s.e.m. ***P<0.001, Student's t-test.