Table 2.
C. trachomatis transcription templates used in this study.
| Plasmid | Promoter (nucleotides relative to transcription start site, +1) |
Reference |
|---|---|---|
| pMT1150 | omcAB promoter region from −122 to +5 | (Wilson & Tan, 2002) |
| pMT1212 | hctB promoter region from −164 to +5 | (Yu & Tan, 2003) |
| pMT1228 | hctB promoter region from −39 to +6 | (Yu et al., 2006a) |
| pMT1232 | tlyC_1 promoter region from −230 to +5 | (Yu et al., 2006b) |
| pMT1234 | pgk promoter region from −266 to +5 | Hilda Yu (unpublished) |
| pMT1236 | tsp promoter region from −273 to +5 | (Yu et al., 2006b) |
| pMT1272 | hctB promoter region from −39 to +6; nucleotide substitution at position −12 from C to A | (Yu et al., 2006a) |
| pMT1273 | hctB promoter region from −39 to +6; nucleotide substitution at position −12 from C to T | (Yu et al., 2006a) |
| pMT1274 | hctB promoter region from −39 to +6; nucleotide substitution at position −12 from C to G | (Yu et al., 2006a) |
| pMT1456 | bioY promoter region from −219 to +5 | (Yu et al., 2006b) |
| pMT1457 | dnaK P2 promoter region from −269 to +5 | (Yu et al., 2006b) |
| pMT1647 | tsp promoter region from −85 to +5 | This work |
| pMT1648 | tsp promoter region from −85 to +5 containing a 10 bp nucleotide substitution in the EUO operator site | This work |
| pMT1662 | dnaK P1 promoter region from −55 to +5 | This work |
| pMT1663 | dnaK P1 promoter region from −55 to +5 containing a 30 bp nucleotide substitution with omcAB EUO-binding operator at positions −26 to +4 in dnaK | This work |