Figure 1. Myeloid cell-derived IL-10 is crucial for survival after Citrobacter rodentium infection.

(a) Il10^−/− (black circle), Il10rb^−/− (gray triangle) and C57BL/6J (black square) mice or (b) Il10^flox/floxLysM-Cre (black circle), Il10^flox/floxCd4-Cre (open circle), Il10^flox/floxCd11c-Cre (black triangle) and Il10^flox/flox (black square) mice were infected orally with C. rodentium and survival was monitored. (a) Two independent experiments with 6 mice per group and (b) 3 independent experiments with 12–17 mice per group are presented. (a,b) Log-Rank test, **p<.01, ***p<.001 (c,d) Inflammation of middle colon was assessed by histology before and 6 days after infection. Scale bars represent 100 μm. (e) Epithelial barrier permeability was determined before and 6 days after infection. (f,g) Titers of C. rodentium in (f) spleen and (g) feces were quantified 6 days of infection. (eg) Each symbol represents a measurement from a single mouse. Student’s t test, **p<.01, ***p<.001 (h) Analysis of Il10 mRNA in macrophages or granulocytes at day 3 after infection. Results were representative of two independent experiments.