Figure 2. Macrophage IL-10 regulates myeloid cell recruitment to the colon.

Il10^flox/floxLysM-Cre (black), Il10^flox/floxCd4-Cre (white) and Il10^flox/flox (grey) mice were analyzed before a day 6 after C. rodentium infection. (a) Lamina propria cells were isolated at the indicated times and total cell numbers were counted. Frequencies of CD4⁺ T cells (CD4⁺TCRβ⁺), monocytes (Ly6G⁻Ly6C^high) and granulocytes (Ly6G^highLy6C^low) were measured by flow cytometry and cell numbers were calculated according to the respective total cell number. Cell numbers are presented as averages and SD from 9 mice per group from 3 independent experiments. (b) At the indicated time points, 3mm fragments of the middle colon of Il10^flox/floxLysM-Cre (black), Il10^flox/floxCd4-Cre (white) mice were cultured for 24h. Chemokine production was measured in the supernatants by Multi-Plex cytokine assay and normalized to the weight of the corresponding tissue fragments. Data are presented as averages with SD from two independent experiments with 6 mice in each group. Student’s t test, * p<.05, ** p<.01, *** p<.001