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. 2015 May 12;10(5):e0127083. doi: 10.1371/journal.pone.0127083

Fig 4. CLR stimulation of cytokine production by BMDCs involves MALT1 protease activity.

Fig 4

BMDCs from Wt, Malt1 -/-, and Malt1 PD/PD mice were stimulated with (A) curdlan to activate Dectin-1, (B) anti-Dectin-2 antibody to activate Dectin-2, and (C) TDB to activate Mincle. IFN-γ, IL-1β, IL-10, IL-12 p70, IL-6, KC (IL-8 homologue), and TNF-α levels were measured after 20 h by MSD, and results are shown only for cytokines for which significant expression was detected. Maximum cytokine concentration ranges detected were: curdlan, 1–3 ng/ml IL-1β, 4–20 ng/ml IL-6, 4–9 ng/ml KC, 0.5–1 ng/ml IL-10, 0.2–0.4 ng/ml IL-12p70, and 40–100 ng/ml TNF-α; anti-Dectin-2, 0.1–0.3 ng/ml IL-6, 0.5–2 ng/ml KC, and 3–15 ng/ml TNF-α; TDB, 0.3–0.6 ng/ml KC, and 0.3–2 ng/ml TNF-α. Where indicated, cells were treated 126 μM Z-VRPR-FMK (VRPR) for 45 min prior to stimulation. All bar graphs represent the average response expressed as a percent of the stimulated Wt control for 6 mice/group, and are representative of 3 or more studies. Significance was determined relative to the stimulated Wt groups, with *p < 0.05, **p < 0.01, †p < 0.001, ‡p < 0.0001. Error bars represent +/- SEM.