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. 2015 May 12;10(5):e0125419. doi: 10.1371/journal.pone.0125419

Fig 2. HCV NS3 induced pro inflammatory cytokine secretion by microglia.

Fig 2

CHME3 cells were exposed to 20 ng/ml of NS3 for different time points and cell culture supernatant was collected for pro inflammatory cytokine ELISA. (A) IL-8 secretion was detected from 3 hr to 24 hr, (B, C, D) IL-6, TNF-α and IL-1βwas detected at 6 and 24 hr time points. The cells were exposed to 2 ng/ml of NS3 for 6 hrs and ELISA was performed for cytokines. (E) The cells secreted significant amount of IL-8, IL-6, TNF-α and IL-1β. The data is expressed as mean (n = 3) ± SE, * p < 0.05, ** p < 0.01.