Fig. 6.
The effects of rimonabant on adipogenesis in hBM-MSCs and PPARγ transactivation. (A) The concentration-dependent effect of rimonabant on adipogenesis in hBM-MSCs was evaluated in 24 well plates. The effect of rimonabant was compared with those of aspirin, glibenclamide and troglitazone. Cell culture supernatants were harvested for the measurement of adiponectin by ELISA at the 7th days after exchanging media containing with IDX. (B) CV-1 cells were transiently cotransfected with the PPARγ expression vector and the PPRE-TK-Luciferase reporter, and then treated with vehicle, rimonabant (RIMO), AEA, NADA and 2-AG (1, 3, and 10 μM) or troglitazone (TRO, 1 μM). Values represent the mean expression ± standard deviation (SD) (n=3). *p≤0.05 and **p≤0.01.