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. Author manuscript; available in PMC: 2016 May 8.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Mar 20;460(3):596–602. doi: 10.1016/j.bbrc.2015.03.075

Figure 1. MT1-MMP activation occurred during S1P- and WSS-stimulated EC invasion in 3D collagen matrices.

Figure 1

(A) Representative photographs of invasion responses. Scale bar,100μm. (B) Western blots are shown from ECs treated with or without 5.3 dyn/cm2 WSS and allowed to invade for 0, 1, 6, 12, and 18hr in the presence of 1μM S1P. Cell extracts were probed with antibodies directed to MT1-MMP (top blot) or GAPDH (bottom blot). Black and white arrowheads indicate pro and active MT1-MMP, respectively in all figures. Densitometric analyses of band intensities for (C) Pro MT1-MMP and (D) Active MT1-MMP levels normalized to GAPDH using ImageJ software from 3 independent experiments (means ±S.D.). In all figures, lowercase letters denote groupings from one-way ANOVA followed by post hoc pairwise comparison testing using Tukey’s method (p<0.05).