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. 2015 May 12;10(5):e0126118. doi: 10.1371/journal.pone.0126118

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© 2015 Schuster et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Native spa promoter, transcript and UACAU sequence mutations. UACAU site is underlined in transcript sequence and mutated bases are marked in pink (mA: insertion, mB: deletion, mC: transversion, mD: transversion to create a VUUV’ sequence). (B) Primer extension results of spa UACAU derivatives subjected to MazF treatment in vivo. Cleavage (arrowhead) of spa was only observed in the UACAU sequence, whereas any changes (including that to VUUV’) abolished cleavage. The shift of the full length transcript band (fuzzy circle) in the mA and mB strains is due to the insertion and deletion of one base each. All plasmids were contained in HG001Δspa to eliminate background from WT spa transcript. gfp stands for pRAB11-gfpmut2; spa WT for the native spa transcript and mA-D for the respective pRAB11 constructs from (A).