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. 2015 May 12;10(5):e0126118. doi: 10.1371/journal.pone.0126118

Fig 6. Cleavage of rsbW transcript in vivo.

Fig 6

(A) native rsb transcript. To increase transcript levels, 30 mM KOH was added to the culture. Slight cleavage bands could be seen at UACAU only in HG003 and the complemented ΔmazEF mutant. (B) Artificially truncated rsbW transcript. 5’ ends of transcripts became visible in both strains upon ATc induction (loading control) due to truncation. Bands indicating cleavage at UACAU were visible in HG003 but not in the ΔmazEF mutant. (C) Artificially truncated rsbW transcript with KOH stress and heat shock. Growth at 42°C increased levels of cleaved rsbW transcript at UACAU.