Table 2.
Concentration and yields of the main fermentation metabolites by the end (262–265 g/L sugar consumed) of fermentations sparged with air or nitrogen in the conditions described in the text
| S. cerevisiae a | M. pulcherrima + 10 % S. cerevisiae a | M. pulcherrima + 1 % S. cerevisiae a | ||
|---|---|---|---|---|
| Glycerol (% w/v) | Airb | 0.83 ± 0.02*A | 1.86 ± 0.18*B | 1.79 ± 0.06B |
| Nitrogenb | 1.20 ± 0.04*A | 1.46 ± 0.06*B | 1.65 ± 0.06C | |
| Ethanol (% v/v) | Airb | 12.9 ± 0.2*B | 11.0 ± 0.3*A | 11.1 ± 0.2*A |
| Nitrogenb | 14.7 ± 0.2* | 13.9 ± 0.6* | 13.9 ± 0.4* | |
| Acetic acid (mg/L) | Airb | 2158 ± 329*B | 676 ± 63*A | 682 ± 123*A |
| Nitrogen | 185 ± 47*B | 63 ± 3*A | 62 ± 2*A | |
| YE/S (g/g) | Airb | 0.384 ± 0.007*B | 0.329 ± 0.010*A | 0.330 ± 0.006*A |
| Nitrogenb | 0.441 ± 0.006* | 0.417 ± 0.014* | 0.416 ± 0.010* | |
| YA/S (mg/g) | Airb | 8.159 ± 1.241*B | 2.553 ± 0.237*A | 2.579 ± 0.461*A |
| Nitrogen | 0.703 ± 0.178*B | 0.238 ± 0.010*A | 0.236 ± 0.007*A | |
| YG/S (g/g) | Airb | 0.031 ± 0.001*A | 0.070 ± 0.007*B | 0.067 ± 0.002B |
| Nitrogenb | 0.045 ± 0.001*A | 0.055 ± 0.002*B | 0.063 ± 0.002C |
Values are shown as mean ± standard deviation of three biological replicates
YE/S ethanol yield on sugar, YA/S acetic acid yield on sugar, YG/S glycerol yield on sugar
aStatistically significant differences (ANOVA) between cultures sparged with air or nitrogen for the same parameter and inoculum are indicated by *
bDifferent capital letters indicate statistically significant differences (ANOVA) for values in the same row