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PC-3 spheroids were exposed to two representative betulin derivatives, 5 and 20, for 4 h (at 1 μM) and 6 days (at 0.3 μM). A) Computational quantification of signal intensities from the kinase arrays, aligned by fold changes observed (left to right). B) Western blot of total and phospho-Akt (S473) shown for both short and long-term exposure to 20 and 5.
