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. Author manuscript; available in PMC: 2015 May 12.
Published in final edited form as: Cold Spring Harb Protoc. 2012 Feb 1;2012(2):150–161. doi: 10.1101/pdb.top067843

Figure 4. Complex Motions of Nuclear Density and patterns.

Figure 4

Stage 5 blastoderm embryos show a complex dynamic pattern of nuclear packing densities. Spatial nuclear densities were calculated for several temporal cohorts of embryos through stage 5 as the number of nuclear centers per μm2. The resulting density maps were computationally unrolled into a 2D projection (A–F). Nuclear densities are represented as a color heat-map with lowest densities (0.025 nuclei/μm2) in blue and the highest densities in red (0.05nuclei/μm2). Isodensity contours were overlayed with expression patterns of sna and eve (G). Expression of pair-rule stripes of ftz and eve vary significantly around the D/V axis (H and I). The stripes show marked differences in expression profiles and have unique modes of expression variation along the D/V axis. The error bars give the 95% confidence intervals for the means. The data were derived from 155 embryos.