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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Virology. 2014 Dec 2;475:139–149. doi: 10.1016/j.virol.2014.10.021

Fig. 4.

Fig. 4

C-terminal mutations within NS4B affect its interaction with NS5A in the two-hybrid system. A. Huh7 cells were transfected with the indicated plasmid combinations. The culture media was collected 48 h post-transfection and tested for SEAP activity as a control for transfection efficiency, followed by a luciferase activity assay performed on cell lysates. Luciferase activity results are presented as a percentage relative to the NS5A and NS4B CTD value. Values are (mean±SD) from triplicate wells. The graph represents three independent experiments. Asterisks denote statistical significance in an unpaired Student t test ***P<0.0005; **P<0.005. B. Expression levels of the mutant NS4B constructs are shown in panel A, as determined by Western blot using GAL4 antibodies. Monoclonal mouse anti-β-actin antibody was used as a loading control. Molecular mass markers are indicated on the right.