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. Author manuscript; available in PMC: 2016 Jun 25.
Published in final edited form as: Neuroscience. 2015 Apr 9;297:211–218. doi: 10.1016/j.neuroscience.2015.03.070

Figure 3.

Figure 3

Atropine induces c-Fos expression in the basal forebrain cholinergic neurons and suppresses c-Fos expression in sleep-active VLPO neurons. Representative photomicrographs of c-Fos (black) and ChAT (brown) double immunostaining in the VLPO and basal forebrain cholinergic neurons of vehicle- (a, b and c) and 100mg/kg atropine (d, e and f) administered rats (b, c, e and f: high-magnification views of the rectangular areas marked in “a”, and “d” respectively). Arrows indicate the c-Fos and ChAT double-stained cells. Scale bars: a and d, 200 μm; b and e, 50 μm; c and f, 25μm. VLPO: ventrolateral preoptic nucleus.