Table 1.
Primer sequences used for cloning of CYP1C1 and CYP1C2 transcripts and quantification of CYP1 gene expression by real time PCR.
| Primer | Sequences |
|---|---|
| Cloning | |
| CYP1C1 Utr F | 5’-ATCAGCACCGAACACCAGCG |
| CYP1C1 Utr R | 5’-CCCCATTCGACTGGATGTTTTAAC |
| CYP1C2 Utr F | 5’-ACCCTCCAACTGAAGAGGGCAGAAA |
| CYP1C2 Utr R | 5’-TCATAGGCAGTGGGTTAGACAGCACA |
| Real time PCR | |
| ZFCYP1A F1) | 5’-GCATTACGATACGTTCGATAAGGAC |
| ZFCYP1A R1) | 5’-GCTCCGAATAGGTCATTGACGAT |
| ZFCYP1B1 F | 5’-GCTCAGCTGGTCCATTGATACC |
| ZFCYP1B1 R | 5’-CATCAGCGACAGCAACACAC |
| ZFCYP1C1 F | 5’-AGTGGCACAGTCTACTTTGAGAG |
| ZFCYP1C1 R | 5’-CCAAACAAGAAAGACTTTTGAGC |
| ZFCYP1C2 F | 5’-GTGGTGGAGCACAGACTAAG |
| ZFCYP1C2 R | 5’-TTCAGTATGAGCCTCAGTCAAAC |
| β-actin F1) | 5’-CAACAGAGAGAAGATGACACAGATCA |
| β-actin R1) | 5’-GTCACACCATCACCAGAGTCCATCAC |
| ARNT2 F | 5’-CACCTTTGGATCACATCTCATTG |
| ARNT2 R | 5’-TCACCCTCCTTAGACGGACC |