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NK4 reduces β-catenin nuclear localization and transcriptional activity. (A) Immunofluorescent staining demonstrating that Ad-NK4 blocks HGF-induced nuclear localization of β-catenin. (B) TOP/FOP luciferase reporter assays performed to determine transcriptional activity of β-catenin. Expression of NK4 inhibited luciferase activity of TOP-Flash but not the negative control vector FOP-Flash in both Hmeso and Meso 10 cell lines (*, p < 0.05).
