Inhibition of peritoneal MM growth by AdNK4. Intraperitoneal xenografts of human Hmeso cells in nude mice were mock-treated (PBS) or treated with Ad-LacZ or Ad-NK4. The treatment was performed twice a week for a total of five administrations. (A) Weight of individual tumors was determined, and bars shown represent mean weight ± s.d. Ad-LacZ versus PBS (*, p > 0.05). Ad-NK4 versus PBS and Ad-LacZ (**, p < 0.01). Images in lower panel depict tumor growth in representative mice. (B) Immunohistochemical staining of tumor tissues. Phospho-Met, phospho-AKT, Oct4, and active β-catenin were markedly reduced in tumors of mice infected with Ad-NK4 compared to that observed for mice treated with PBS or Ad-LacZ. Photographs were taken at ×400 magnification.