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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 2;1853(7):1626–1635. doi: 10.1016/j.bbamcr.2015.03.016

Figure 5. p70S6 kinase synergizes Akt1 and Mek1-mediated synthesis and secretion of ECM proteins by the fibroblasts.

Figure 5

(A) Western blot images of cell lysates and conditioned media from either NIH 3T3 fibroblasts expressing either CA-Akt1 or DN-Mek1 and/or co-treated with Rapamycin (mTOR inhibitor) or PF4708671 (p70S6 Kinase inhibitor), followed by treatment with DMSO or 100 pM TGFβ in serum free medium. (B) Bar graphs showing densitometry analysis of cell lysates and conditioned media for the synthesized and secreted fibronectin and collagen I expressing either CA-Akt1 or DN-Mek1 and/or co-treated with Rapamycin or PF4708671, followed by treatment with DMSO or 100 pM TGFβ in serum free medium. Data presented as mean ± SD (*p<0.001; Δp<0.01; #p<0.05; n=3).