Figure 5. Time of day influences voiding behavior in adult male mice.
(A) Representative images of spontaneous void assay testing in the same adult virgin male mice. Mice were tested in the morning (9am–1pm), afternoon (2pm–6pm) or night (10pm–2am) over a one week period. Quantification of (B) urine area, (C) primary void area, (D) body mass, (E) urine area normalized to body mass and (F) primary void area normalized to body mass. Results are mean±SE, n=8. Asterisk indicates significant differences p≤0.05.