Table 2. Fertilization and embryo development of vitrified-warmed germinal vesicle stage (GV) oocytes after in vitro fertilization.
| Experimental Groups | GV | Oocytes | Oocytes | Embryos | Blastocysts | Cell number (Mean±SEM) | ||
|---|---|---|---|---|---|---|---|---|
| oocytes | survived | fertilized | cleaved | Total | Trophectoderm | Inner cell mass | ||
| n | n, (%) | n, (%) | n, (%) | n, (%) | (n) | |||
| Control-Fresh | 191 | 191 | 158 | 118 | 55 | 122.0±6.9 | 91.1±5.5 | 30.9±2.4 |
| (100) a | (84.4±2.1) a | (65.0±3.4) a | (27.9±2.4) a | (n = 16) | ||||
| Control-Vitrification | 214 | 199 | 118 | 56 | 13 | 106.8±10.8 | 81.0±8.9 | 25.8±2.5 |
| (90.9±2.0) b | (55.9±4.8) b | (27.0±3.7) b | (6.2±2.2) b | (n = 6) | ||||
| FSK-Fresh | 177 | 177 | 139 | 103 | 53 | 122.5±5.5 | 92.5±4.7 | 30.1±1.6 |
| (100) a | (78.2±2.6) a | (59.9±2.8) a | (29.9±6.9) a | (n = 20) | ||||
| FSK-Vitrification | 216 | 200 | 128 | 86 | 39 | 118.8±7.9 | 88.7±6.1 | 30.1±2.3 |
| (92.3±0.9) b | (60.4±5.4) b | (40.3±4.7) c | (18.6±5.9) a,b | (n = 16) | ||||
| IBMX-Fresh | 176 | 176 | 133 | 105 | 55 | 118.6±6.3 | 89.0±4.6 | 29.5±2.1 |
| (100) a | (75.0±2.2) a | (59.7±1.7) a | (30.2±6.3) a | (n = 22) | ||||
| IBMX-Vitrification | 216 | 197 | 121 | 79 | 36 | 120.2±7.7 | 90.2±6.4 | 30.0±2.0 |
| (90.6±1.2) b | (56.2±3.6) b | (36.5±4.9) b,c | (17.9±6.5) a,b | (n = 18) | ||||
| FSK/IBMX-Fresh | 50 | 50 | 17 | 14 | 9 | 123.9±10.6 | 92.3±7.5 | 31.6±3.3 |
| (100) a | (35.8±9.2) c | (30.0±1.0) b,c | (18.3±1.7) a,b | (n = 9) | ||||
| FSK/IBMX-Vitrification | 50 | 45 | 11 | 9 | 7 | 111.6±10.5 | 84.0±8.6 | 27.6±3.0 |
| (90.0±0.2) b | (23.3±6.7) c | (19.2±5.8) d | (14.2±0.8) b | (n = 7) | ||||
Values with different superscripts (a, b, c, d) within each column are significantly different from each other (P < 0.05).