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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mucosal Immunol. 2014 Nov 12;8(4):785–798. doi: 10.1038/mi.2014.109

Figure 8. IL-13 and NGF/NTRK1 synergistically stimulate CCL26 production.

Figure 8

In A–D, transcriptional levels of CCL26, SERPINB4, KITLG and ID3 after IL-13 and/or NGF stimulation of TE-7 cells stably expressing NTRK1 were measured by RT-PCR. Induction was performed for 6 hr, IL-13 concentration was 1 ng/ml, NGF was used at 100 ng/ml. Expression was normalized to the level of GAPDH. In E, CCL26 protein level in cell supernatant was measured by ELISA. Cells were stimulated with IL-13 and NGF (100 ng/ml) for 24 hr; mean fold induction relative to untreated cells is shown. Error bars represent standard error of the mean for 4 independent experiments. In F, shown is the relative level of expression of the indicated genes following down regulation of EGR1 by siRNA in TE-7 cells. Expression of genes in cells transfected with control siRNA was set as 1 (dotted line). Data for 4 to 5 independent experiments are presented as box and whiskers plot; ****p < 0.0001, ***p < 0.001, **p < 0.01, ns, not significant.