Skip to main content
. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Virology. 2004 Oct 10;328(1):74–88. doi: 10.1016/j.virol.2004.07.019

Fig. 4.

Fig. 4

Cloning and characterization of the R3 envelopes. (A) HTA analysis of the parental viruses and the clones obtained by nested PCR. PBMCs infected with the R3 viruses were used for nested PCR and products were cloned into a retroviral vector. PCR was performed on the V1/V2 region of the original viruses or of the clones. Resulting products were hybridized to the radiolabeled V1/V2 probe from the JR-FL virus. Heteroduplexes were visualized on polyacrylamide sequencing gels. Envelope functionality was determined by infecting SupT1 cells with pseudotyped virus. NA = not applicable, ND = not done. (B) Expression and processing of the envelope clones were assessed after transfection into A293T cells and probing of lysates with a polyclonal anti-gp120 antibody. (C) Sequence of the R3A and R3B envelopes. The V1-V5 regions and the fusion domains are indicated and the transmembrane domain of gp41 is shown in bold. The space in the V5 region of the R3B sequence indicates a gap.