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. 2015 May 12;10(5):e0127222. doi: 10.1371/journal.pone.0127222

Fig 2. Expression of PTGDS and Nptx2 in primary OPC.

Fig 2

A Expression of cell-type specific markers was analyzed by Western-Blot of total cell lysates of pOPC and the negative (neg.) sort fractions, HEK cells and primary cortical neurons (DIV5 in culture). B-D show the expression of proteins in pOPC over time, related to DIV0. In B a peak of the OPC protein NG2 is shown at DIV1, PLP indicates differentiation into oligodendrocytes starting at DIV2, GFAP shows astrocyte differentiation starting at DIV2. C PTGDS expression peaks together with NG2 at DIV1. D Nptx2 expression increases together with NG2 at DIV1, peaks at DIV2 and returns to basal levels at DIV4. E Expression of PTGDS and Nptx2 mRNA in pOPC at DIV1, as revealed by in situ hybridization, OPC were identified by antibody staining of NG2 protein. (A-D: 3 sorts were analyzed for each time point. Two tailed student’s t-test was applied.)