Fig 5. Western blots presenting quantitative analysis of the RnhA protein in protein extracts of M. smegmatis.
The upper part of the figure presents detection of either LigA or RnhA in protein extracts isolated from mutants used in this study. The lower part of the figure presents quantitative analysis of the amount of RnhA in M. smegmatis mc2 155. Briefly, we detected known concentrations of either recombinant LigA or recombinant RnhA. Further, we compared the intensities of the obtained bands with those obtained from various concentrations of total protein extracts of M. smegmatis mc2 155. We were able to calculate, that RnhA is approximately 133 times less abundant that LigA.