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. 2015 May 12;10(5):e0126260. doi: 10.1371/journal.pone.0126260

Fig 8. Constitutive stable DNA replication of E. coli ∆rnhA mutants.

Fig 8

An alternative mode of initiation of DNA replication was discovered in E. coli mutants lacking RNase H type I encoded by rnhA. (A,B) The initial strand opening involves RecA dependent hybridization of the RNA transcript to dsDNA, (C) The resulting R-loop is not resolved by RNase HI and therefore RNA persists on DNA strand and serves as a primer for elongation by PolI, (D,E) When the loop opens sufficiently, primosome is loaded on the leading strand. As replication continues, PolI removes persisting RNA transcript and primosome is loaded on the lagging strand.