A) MUC16 Transcript Number. The Cancer Genome Atlas ovarian cancer project is a well-studied collection that contains 316 serous ovarian cancers with complete data, including clinical outcome data. Since expression of MUC16 protein is an important driver of cancer behavior in ovarian cancer, we examined the impact of MUC16 copy number on MUC16 mRNA expression. The MUC16 transcript expression was related to the number of MUC16 gene copies, although there was a broad variation in MUC16 transcript expression in all of the groups examined (except, of course, the rare homozygous deletion of MUC16). In most cases, the MUC16 mRNA expression was clustered at higher transcript numbers than the normal fallopian tube samples included as controls. B) The quintile of patients with the highest MUC16 expression, combined with the 18 patients with identified MUC16 mutations, have a significantly (p = 0.02117) worse survival compared to the patients with lower MUC16 expression in a Kaplan-Meier analysis. C) An OncoPrint showing the relationship of MUC16 genetic alterations with PI3K mutational events in ovarian cancer.