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Heparin displacement of (A) siRNA/mPEG-b-P(APNBMA)23.6 polyplexes (N/P = 4), and (B) siRNA/PEI polyplexes (N/P = 6) incubated in the presence of increasing amounts of heparin. (C) Quantification of free siRNA in siRNA/mPEG-b-P(APNBMA)23.6 polyplexes (black) and siRNA/PEI polyplexes (white) based on gel image analysis using ImageJ.
