Table 1.
Primers used in the RT-PCR and sequencing. The position numbering is according to the EBLV-2 isolate RV1333 (EF157977)
| Primer | Sequence (5’-3’) | Position |
|---|---|---|
| 1f | acg ctt aac gac aaa acc ag | 1 - 20 |
| 1r | tag ctc tcc caa tcg tca gg | 326 - 345 |
| 2f | cgc tag gtt gga tcc tga tg | 256 - 275 |
| 2r | ggc gca cat ctt gtg agt ag | 636 - 655 |
| 3f | cca acg tag ctg aca gaa tgg | 558 - 578 |
| 3r | aca tct cgt gag gtg cac ag | 1066 - 1085 |
| 4f | cgg gag tta cat ggg tca ag | 1015 - 1034 |
| 4r | gtc tgg cct gat gat tcg ag | 1354 - 1373 |
| 5f | cag gat cat ggt caa tgg | 1291 - 1308 |
| 5r | tcc caa cac cct caa ggt ag | 1812 -1831 |
| 6f | aag aag aag gaa gcg atg agg | 1745 - 1765 |
| 6r | tgc gct att tct gct tca ac | 2116 - 2135 |
| 7f | acc tgc gct gga atg gtc | 2070 - 2087 |
| 7r | ggg agc cat agg tca tca tc | 2591 - 2610 |
| 8f | agt gag agg ttg cag gga tg | 2530 - 2549 |
| 8r | act ctg ccc att gaa aca cc | 2869 - 2888 |
| 9f | ttc cag agg gaa tga act gg | 2826 - 2845 |
| 9r | ggt gtt cag tcg ggt gtt tc | 3245 - 3264 |
| 10f | ctt tta tga gca ata gaa caa aac c | 3186 - 3210 |
| 10r | atg tcg gat cac ctg cag tc | 3688 - 3704 |
| 11f | aac tac cac gtt caa gag aaa gc | 3619 - 3641 |
| 11r | ttt gcc tca tcg tga ttt tg | 4115 - 4134 |
| 12f | tga aac tgt gtg gaa tct ctg g | 4059 - 4080 |
| 12r | atg ctg ttg aag cat tgc ag | 4518 - 4537 |
| 13f | tcc tca tca caa tgg agt ctt c | 4441 - 4462 |
| 13r | ccc act ttg gga agt gac ag | 4830 - 4849 |
| 14f | aaa gag agc caa acc caa cc | 4787 - 4805 |
| 14r | att gca tcc tct ccc act tg | 5154 - 5171 |
| 15f | acc ggt aca cag ggt ctt gc | 5076 - 5095 |
| 15r | gca tct atc tcc ggt tcg ac | 5458 - 5477 |
| 16f | aga tga ttg atc ccc tgg ag | 5414 - 5433 |
| 16r | gag gca ctt tcg act tct gg | 5747 - 5766 |
| 17f | cgc aca atc cat gat gtc tc | 5697 - 5716 |
| 17r | gaa tca gga ggg agt tga acc | 6173 - 6193 |
| 18f | tct cag agt gcc aac tgt ctg | 6106 - 6126 |
| 18r | gtt cct tca agc tgg ctc ac | 6415 - 6434 |
| 19f | tta gtg cag agg gct gaa gg | 6340 - 6359 |
| 19r | tat ggg atc aaa ggg tgg tc | 6709 - 6728 |
| 20f | ctg gct aaa cgg atc ctc ag | 6634 - 6653 |
| 20r | aag aat tcc ctg ggg ttg ac | 6964 - 6983 |
| 21f | ccg tcc cca gtg aga aag tc | 6917 - 6936 |
| 21r | gac ctt gtc ccg tga ctc tg | 7209 - 7228 |
| 22f | ttg gcg aac tac atc tta ccc | 7129 - 7149 |
| 22r | tga gtc cct ctt ggg tca ac | 7641 - 7660 |
| 23f | agc aca ggg aga caa cca ag | 7590 - 7609 |
| 23r | gtg aaa tac cgc ctg gac tg | 7979 - 7998 |
| 24f | gtc gca cag cat tca caa tc | 7924 - 7943 |
| 24r | agc aga atg gtt gga ctt gc | 8332 - 8351 |
| 25f | ccg gac ttg ggt gat aga ag | 8254 - 8273 |
| 25r | aaa ttg ccg tcg aat tgt tc | 8567 - 8586 |
| 26f | gct cat cct tcc tcg gaa tac | 8513 - 8533 |
| 26r | gat ttg agt ccc tgg caa tg | 9021 - 9040 |
| 27f | cca acg tcc atg ttg tca ag | 8966 - 8985 |
| 27r | aga cat ccg gga aca tga ag | 9417 - 9436 |
| 28f | caa gtg cat ccg acc gat ag | 9369 - 9388 |
| 28r | cag atc gaa gtg agg gtt cc | 9831 - 9850 |
| 29f | tgt tga ggc tag aca atc atc c | 9788 - 9809 |
| 29r | taa ggt gtc ttc ccc gtg ac | 10151 - 10167 |
| 30f | atc cga ctc agg cag ttg ag | 10105 - 10124 |
| 30r | gag gcc atg agg tca ttc ac | 10606 - 10625 |
| 31f | tgg aat ctc cag aac tgt gc | 10539 - 10558 |
| 31r | tgg cct tgt agt ctg ggt tc | 10923 - 10942 |
| 32f | ctc tcg atc aat ggt cca ctc | 10867 - 10887 |
| 32r | tta gcc aag gtc cct ctt tg | 11287 - 11306 |
| 33f | tga agt cga gtc att cct agt cc | 11238 - 11260 |
| 33r | gct act acc ggc aag tcg ag | 11672 - 11691 |
| 34f | aag caa gtc ata cga gga ag | 11533 - 11552 |
| 34r | acg ctt aac aaa aaa aac ata g | 11909 - 11930 |