Figure 9.

Relief memory and punishment memory remain impaired in control conditions without acute and local restoration of Synapsin. A, Expression pattern of Synapsin in uninduced control flies of the indicated genotypes. In the bottom rows (Merge), anti-Synapsin staining in brains and thoracic nervous systems is shown in green, while cell-body counterstaining with propidium iodide is shown in magenta. The mushroom body region of A1–4 is shown at higher magnification in A9–12. The stippled line in A9 and A10 indicates the mushroom body neuropil. The mushroom body region of A21–24 is shown at higher magnification in A29–32. Note the absence of anti-Synapsin staining in A10 and A30. Scale bars: 100 μm. B, C, In uninduced control conditions, relief memory (B) and punishment memory (C) remain abolished in the experimental genotype. *p < 0.05/3 and ns: p > 0.05/3 are used for pairwise comparisons. Gray shading of the boxes indicates significance from chance (zero) at p < 0.05/4.