. 2015 May 13;35(19):7487–7502. doi: 10.1523/JNEUROSCI.4454-14.2015

Table 1.

Phosphosites of the Synapsin protein determined by LC-MS/MS

Amino acid	Phosphosite in Synapsin isoform D E2QCY9_DROME 1041 AS	Phosphosite in Synapsin Q24546_DROME 1025 AS^c	Frequency of detection (n = 85)	P value	Predicted kinases (NetPhosK-Score)	Lit.
S^a	22	6	1	6.31E-07	PKA, 0.85
					RSK, 0.65
T	86	70	40	7.94E-04	p38MAPK, 0.53
T	89	73	43	1.26E-03
S	99	83	6	2.00E-03	PKA, 0.66
					PKG, 0.59
T	102	86	0	c.a.n.p^b	PKC, 0.79
					cdc2, 0.50	Nuwal et al., 2011
					cdk5, 0.75
S	103	87	4	2.51E-03	p38MAPK, 0.58
					GSK3, 0.53	Nuwal et al., 2011
S	107	91	38	2.00E-03
S	117	101	4	2.00E-06	RSK, 0.57
S	128	112	25	1.58E-03	cdc2, 0.50
S	136	120	11	2.00E-03
T	138	122	37	1.58E-03	PKC, 0.76
T	195	179	7	1.58E-03
T	200	184	11	1.26E-03
Y	204	188	8	1.26E-03
T	208	192	4	1.58E-03	PKC, 0.71
					cdk5, 0.65
S	480	464	81	5.01E-10	RSK, 0.55
					GSK3, 0.51	Nuwal et al., 2011
T	482	466	26	2.51E-09	CKII, 0.52	Nuwal et al., 2011
					cdk5, 0.62
T	489	473	1	1.58E-04	p38MAPK, 0.53
					GSK3, 0.51
S	512	496	24	1.00E-03
S	522	506	2	1.26E-08	PKC, 0.79
					PKA, 0.52
S	523	507	4	5.01E-10	RSK, 0.59
S	525	509	2	1.26E-08		Zhai et al., 2008
S	526	510	18	7.94E-12	PKA, 0.67
					CKI, 0.60	Zhai et al., 2008
S	549	533	29	1.00E-11	PKA, 0.81
					DNAPK, 0.61
					RSK, 0.60
T	551	535	56	7.94E-14	PKC, 0.78
S	554	538	0	c.a.n.p^b		Nuwal et al., 2011
S	725	709	26	1.58E-03
T	728	712	17	3.16E-04	PKC, 0.51
S	752	736	5	5.01E-04
S	952	936	3	3.98E-07	CKI, 0.50
S	977	961	0	c.a.n.p^b	cdc2, 0.50	Nuwal et al., 2011
Y	998	982	0	c.a.n.p^b		Nuwal et al., 2011

^aOnly in the edited version of the protein was a phosphorylation at this site detected.

^bc.a.n.p, covered as nonphosphorylated (peptides containing this motive were found as nonphosphorylated only).

^cFor convenience we added amino acid numbering as reported previously (Zhai et al., 2008; Nuwal et al., 2011) using the matrix (Q24546_DROME; http://www.uniprot.org).