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. 2015 May 13;35(19):7643–7653. doi: 10.1523/JNEUROSCI.4724-14.2015

Figure 2.

Figure 2.

NSF content is reduced in BLOC-1 deficiency. A, B, Downregulation of BLOC-1 subunits decreases NSF content in neuroblastoma cells. C, Quantification of data in A and B. D–H, We used two neuroectodermal-derived cells, MNT1 melanoma cells, and immortalized melanocytes from BLOC-1-null mice. Loss of BLOC-1 reduced the content of NSF in both cells. Controls were the mutant melanocytes rescued by expression of the missing BLOC-1 gene (lane 1). G presents the quantification of D–F. H presents quantitative RT-PCR data measuring transcript levels of NSF and VAMP2 from immortalized melanocytes from BLOC-1-null mice and the above-described controls. All p values <0.05, unless otherwise indicated, as not significant in H (NS), Wilcoxon–Mann–Whitney rank sum test. Dots represent independent biological replicates.