Skip to main content
. 2012 Feb;180(2):831–838. doi: 10.1016/j.ajpath.2011.10.007

Figure 2.

Figure 2

Binding of Ets-1 to an enhancer sequence for the JunB promoter in HL and ALCL cell lines. EMSA analysis of Ets-1 binding to an enhancer sequence for the JunB promoter using 2 μg of nuclear extracts of HL cell lines (L428, KMH2, L540, and HDLM2) and ALCL cell lines (Karpas299 and SUDHL1) (A). Competition analysis using a maximum 200-fold molar excess of the unlabeled probe containing the Ets-1–PEA3 consensus sequence (B), and supershift analysis using the anti-Ets-1 antibody (2 μg) (C), was performed using the SUDHL1 ALCL cell line. D: ChIP analysis. DNA immunoprecipitated with the anti-Ets-1 antibody was assayed by PCR using two sets of primer pairs for the JunB promoter (A and B) and was analyzed by agarose gel electrophoresis. Immunoprecipitation of SUDHL1 DNA with an anti-RNA polymerase II antibody and an isotype-matched IgG antibody served as positive and negative controls, respectively. Direct amplification of SUDHL1 DNA also served as a positive control and is indicated as input.