Figure 1.

Expression of mutant BRAF (V600E) suppresses cellular proliferation and induces pCHK2 and γH2AX nuclear foci. Forty-eight hours after transfecting epithelial cells, robust expression levels of wild-type and mutant BRAF can be detected in cyst108 cells (A) and in RK3E cells (B). A reduced cellular proliferation was recorded in cyst108 cells (C) and in RK3E cells (D) that expressed mutant BRAF. Forty-eight hours after transfection, cells were stained for pCHK2 and γH2AX. Nuclear foci for pCHK2 and γH2AX immunofluoresence were observed in mutant BRAF-expressing cyst108 cells (E) and RK3E cells (F). UV light-treated cells serve as the positive control for immunofluoresence staining. MT, mutant; WT, wild type.