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. 2015 May 12;5:87. doi: 10.3389/fonc.2015.00087

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Copyright © 2015 Gorchs, Hellevik, Bruun, Camilio, Al-Saad, Stuge and Martinez-Zubiaurre.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of T-cell derived immunogenic cytokines secretion by conditioned medium from irradiated and control CAFs. Quantitative levels of (A) IFN-γ and (B) TNF-α were measured by multiplex cytokine immune-assays in the supernatants of T-cell cultures. Experimental groups include PBMCs in regular growth medium or PBMCs in CM obtained from irradiated (iCAF-CM) and non-irradiated (CAF-CM) CAF cultures. Data represent mean ± SD of five different CAF donors from triplicate experiments. Student’s t-test value (***p < 0.0001). ND; non-detected.