Table 3.
Bacterial viability determination from untreated and heat-fixed kidney tissue obtained from a tissue mimetic model
Bacterial isolate | Treatment | 1:10 | 1:100 | 1:1000 | cfu/ml |
---|---|---|---|---|---|
E.coli Top10 | none | TNTC | 1000+ | 1000+ | >103 |
E.coli pGSV3-GmR | none | TNTC | 1000+ | 1000+ | >103 |
E.coli pAM17-KanR | none | TNTC | 1000+ | 1000+ | >103 |
Klebsiella pneumoniae | none | TNTC | 1000+ | 1000+ | >103 |
Burkholderia thailandiensis | none | TNTC | 1000+ | 1000+ | >103 |
Acinetobacter baumani | none | TNTC | 1000+ | 1000+ | >103 |
Control (no bacteria) | none | 0 | 0 | 0 | 0 |
E.coli Top10 | heat fixed | 0 | 0 | 0 | 0 |
E.coli pGSV3-GmR | heat fixed | 0 | 0 | 0 | 0 |
E.coli pAM17-KanR | heat fixed | 0 | 0 | 0 | 0 |
Klebsiella pneumoniae | heat fixed | 0 | 0 | 0 | 0 |
Burkholderia thailandiensis | heat fixed | 0 | 0 | 0 | 0 |
Acinetobacter baumani | heat fixed | 0 | 0 | 0 | 0 |
Control (no bacteria) | heat fixed | 0 | 0 | 0 | 0 |
TNTC = too numerous to count, HF = Heat fixed.