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. 2015 May 8;5:10320. doi: 10.1038/srep10320

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Copyright © 2015, Macmillan Publishers Limited

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Measured values of V_rev at ΔpH –1.0, 0, or 1.0 (mean ± SEM, n = 3, 9, or 6, respectively), with pH_o ranging 5.5 to 7.0 and pH_i ranging 5.5 to 8.0. The linear regression slope was 53.3 mV/unit ΔpH, compared with the Nernst value of 58.4 mV. Inset: Proton currents in an inside out patch during pulses applied in 5 mV increments (left) indicate reversal between 0 and 5 mV (the conductance activated negative to V_rev) at pH_i 7.0, with pH_o 7.0 (in the pipette). Tail currents in the same patch at pH_i 6.0 indicate reversal at –58 mV. Both values are near the Nernst predictions of 0 mV and –58.4 mV.