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. 2015 May 9;16(1):366. doi: 10.1186/s12864-015-1539-8

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© Haçarız et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nonsynonymous/synonymous substitution rate of the liver fluke transcripts. Ka/Ks ratio distribution (%) of a total of 12,394 Dugesiidae or C. elegans orthologous F. hepatica transcripts is shown. The majority of the transcripts [89.63% (87.90% at P < 0.05)] were found to be associated with negative selection (Ka/Ks < 1). A group of transcripts [6.75% (1.88% at P < 0.05)] could be under directional selection (0.5 < Ka/Ks < 1). Another group of transcripts (0.24%) were related to neutral selection. Only a small portion of the transcripts (3.37%, n = 418) showed the signs of positive selection (Ka/Ks > 1) and, of this, a minority (0.64%, n = 79) reached statistical significance (P < 0.05).