Table 5. Detailed information regarding the primers used for qRT-PCR variation.
| Gene | Forward primer(5'-3') | Reverse primer(5'-3') | Tm (°C) | Product (bp) |
|---|---|---|---|---|
| Cucsa.078110.1 | CGGTGTCGGAGGCAGTGAA | GGCGGAATTGAAGGTTGA | 62.3(56.3) | 197 |
| Cucsa.153420.1 | TCGTGCTGGTGCTAAACT | GAGGCTTGAGCTAGGATGT | 51.6(51.4) | 222 |
| Cucsa.193360.1 | AAAGAGCTATTCCGATGAC | AAAGATGAAGAGGGTAACAA | 49.1(49.0) | 122 |
| Cucsa.218550.1 | GAAATGGTTCGGGATAAT | TTGTTGGTTGCTGCTAAT | 49.3(49.1) | 189 |
| Cucsa.283380.1 | GCTTCACCCATTACACCG | CCAACGCTAACACCACAA | 53.9(52.7) | 172 |
| Cucsa.176670.1 | AACGAATCAGCACCTCAG | GTTCTCACCTCGCAATCT | 50.5(49.5) | 189 |
| Cucsa.057870.1 | GTTCATAAATTGTGGAGGAC | TTGGTAGATGTTCGGGAT | 49.1(50.0) | 196 |
| Cucsa.155940.1 | GCCTCGCCAAGGATTTAG | GCCGCTCCACTGACAGAT | 55.6(55.9) | 189 |
| Cucsa.383730.1 | AATCTTCTGCCACGACAA | TCATCTCCTTCATTACGC | 51.1(48.0) | 239 |
All raw sequencing data have been submitted to European Nucleotide Archive (https://www.ebi.ac.uk/ena), The archive number is PRJEB8421. But the data and metadata will be held private until '05-Apr-2015', except the data is published in a journal.