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. 2015 Apr 24;8:245. doi: 10.1186/s13071-015-0836-z

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© Gumiel et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Rarefaction curves of 16S rDNA sequences from triatomine gut microbiota. The rarefaction curves were calculated using the RDP pipeline in libraries of T. brasiliensis (25Tb and 35Tb) and T. pseudomaculata (17Tp and 19Tp) 16S rDNA fragments. Panel A: The number of different bacterial species is given as a function of the number of sequences obtained by pyrosequencing. Panel B: In contrast to T. pseudomaculata (19Tp) that does not reach species saturation even with the sequencing of 5,000 sequences, the microbiota of T. brasiliensis (25Tb and 35Tb) and T. pseudomaculata (17Tp) approached saturation with less than 100 sequences. The OTU numbers are referred to 0.05.